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J. Virol., 03 1997, 1842-1849, Vol 71, No. 3
Copyright © 1997, American Society for Microbiology

Construction of adenovirus vectors through Cre-lox recombination

S Hardy, M Kitamura, T Harris-Stansil, Y Dai and ML Phipps
Somatix Therapy Corporation, Alameda, California 94501-1034, USA. shardy@apple.somatix.com

Two barriers prevent adenovirus-based vectors from having wide application. One is the difficulty of making new adenoviruses, and the second is the strong immunological reaction to viral proteins. Here we describe uses of Cre-lox recombination to overcome these problems. First, we demonstrate a simple method for constructing E1-substituted adenoviruses. Second, we demonstrate a method to construct adenovirus vectors carrying recombinant genes in place of all of the viral genes, so-called gutless adenovirus vectors. The pivotal feature in each method is the use of a negatively selected adenovirus named psi5. We engineered a cis-acting selection into psi5 by flanking its packaging site with loxP sites. When psi5 was grown in cells making a high level of Cre recombinase, the packaging site was deleted by recombination and the yield of psi5 was reduced to 5% of the wild-type level. To make a new E1-substituted virus, we used psi5 as a donor virus and recombined it with a shuttle vector via a loxP site. The resulting recombinant virus has a single loxP site next to the packaging site and therefore outgrows psi5 in the presence of Cre recombinase. To make a gutless virus, we used psi5 as a helper virus. The only viral sequences included in the gutless vector are those needed in cis for its replication and packaging. We found that a loxP site next to the packaging site of the gutless virus was necessary to neutralize homologous recombination between psi5 and the gutless viruses within their packaging domains.


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  • Shieh, J.-J., Pan, C.-J., Mansfield, B. C., Chou, J. Y. (2004). A Potential New Role for Muscle in Blood Glucose Homeostasis. J. Biol. Chem. 279: 26215-26219 [Abstract] [Full Text]  
  • Everett, A. D., Narron, J. V., Stoops, T., Nakamura, H., Tucker, A. (2004). Hepatoma-derived growth factor is a pulmonary endothelial cell-expressed angiogenic factor. Am. J. Physiol. Lung Cell. Mol. Physiol. 286: L1194-L1201 [Abstract] [Full Text]  
  • Song, B. D., Yarar, D., Schmid, S. L. (2004). An Assembly-incompetent Mutant Establishes a Requirement for Dynamin Self-assembly in Clathrin-mediated Endocytosis In Vivo. Mol. Biol. Cell 15: 2243-2252 [Abstract] [Full Text]  
  • Ghosh, A., Shieh, J.-J., Pan, C.-J., Chou, J. Y. (2004). Histidine 167 Is the Phosphate Acceptor in Glucose-6-phosphatase-{beta} Forming a Phosphohistidine Enzyme Intermediate during Catalysis. J. Biol. Chem. 279: 12479-12483 [Abstract] [Full Text]  
  • Aleyasin, H., Cregan, S. P., Iyirhiaro, G., O'Hare, M. J., Callaghan, S. M., Slack, R. S., Park, D. S. (2004). Nuclear Factor-{kappa}B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24: 2963-2973 [Abstract] [Full Text]  
  • Nwanegbo, E., Vardas, E., Gao, W., Whittle, H., Sun, H., Rowe, D., Robbins, P. D., Gambotto, A. (2004). Prevalence of Neutralizing Antibodies to Adenoviral Serotypes 5 and 35 in the Adult Populations of The Gambia, South Africa, and the United States. CVI 11: 351-357 [Abstract] [Full Text]  
  • Ma, H., Sumbilla, C. M., Farrance, I. K. G., Klein, M. G., Inesi, G. (2004). Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes. Am. J. Physiol. Cell Physiol. 286: C556-C564 [Abstract] [Full Text]  
  • Nemunaitis, J., Sterman, D., Jablons, D., Smith, J. W. II, Fox, B., Maples, P., Hamilton, S., Borellini, F., Lin, A., Morali, S., Hege, K. (2004). Granulocyte-Macrophage Colony-Stimulating Factor Gene-Modified Autologous Tumor Vaccines in Non-Small-Cell Lung Cancer. JNCI J Natl Cancer Inst 96: 326-331 [Abstract] [Full Text]  
  • Kraft, A. D., Johnson, D. A., Johnson, J. A. (2004). Nuclear Factor E2-Related Factor 2-Dependent Antioxidant Response Element Activation by tert-Butylhydroquinone and Sulforaphane Occurring Preferentially in Astrocytes Conditions Neurons against Oxidative Insult. J. Neurosci. 24: 1101-1112 [Abstract] [Full Text]  
  • Tuettenberg, A., Jonuleit, H., Tuting, T., Bruck, J., Biermann, V., Kochanek, S., Knop, J., Enk, A. H. (2004). Early Adenoviral Gene Expression Mediates Immunosuppression by Transduced Dendritic Cell (DC): Implications for Immunotherapy Using Genetically Modified DC. J. Immunol. 172: 1524-1530 [Abstract] [Full Text]  
  • Hinnebusch, B. F., Siddique, A., Henderson, J. W., Malo, M. S., Zhang, W., Athaide, C. P., Abedrapo, M. A., Chen, X., Yang, V. W., Hodin, R. A. (2004). Enterocyte differentiation marker intestinal alkaline phosphatase is a target gene of the gut-enriched Kruppel-like factor. Am. J. Physiol. Gastrointest. Liver Physiol. 286: G23-G30 [Abstract] [Full Text]  
  • Stilwell, J. L., McCarty, D. M., Negishi, A., Superfine, R., Samulski, R. J. (2003). Development and Characterization of Novel Empty Adenovirus Capsids and Their Impact on Cellular Gene Expression. J. Virol. 77: 12881-12885 [Abstract] [Full Text]  
  • Shieh, J.-J., Pan, C.-J., Mansfield, B. C., Chou, J. Y. (2003). A Glucose-6-phosphate Hydrolase, Widely Expressed Outside the Liver, Can Explain Age-dependent Resolution of Hypoglycemia in Glycogen Storage Disease Type Ia. J. Biol. Chem. 278: 47098-47103 [Abstract] [Full Text]  
  • Song, J. J., Lee, Y. J. (2003). Role of the ASK1-SEK1-JNK1-HIPK1 Signal in Daxx Trafficking and ASK1 Oligomerization. J. Biol. Chem. 278: 47245-47252 [Abstract] [Full Text]  
  • Wang, X., He, K., Gerhart, M., Jiang, J., Paxton, R. J., Menon, R. K., Black, R. A., Baumann, G., Frank, S. J. (2003). Reduced Proteolysis of Rabbit Growth Hormone (GH) Receptor Substituted with Mouse GH Receptor Cleavage Site. Mol. Endocrinol. 17: 1931-1943 [Abstract] [Full Text]  
  • Luo, H. R., Hattori, H., Hossain, M. A., Hester, L., Huang, Y., Lee-Kwon, W., Donowitz, M., Nagata, E., Snyder, S. H. (2003). Akt as a mediator of cell death. Proc. Natl. Acad. Sci. USA 100: 11712-11717 [Abstract] [Full Text]  
  • Lee, J.-M., Shih, A. Y., Murphy, T. H., Johnson, J. A. (2003). NF-E2-related Factor-2 Mediates Neuroprotection against Mitochondrial Complex I Inhibitors and Increased Concentrations of Intracellular Calcium in Primary Cortical Neurons. J. Biol. Chem. 278: 37948-37956 [Abstract] [Full Text]  
  • Soiffer, R., Hodi, F. S., Haluska, F., Jung, K., Gillessen, S., Singer, S., Tanabe, K., Duda, R., Mentzer, S., Jaklitsch, M., Bueno, R., Clift, S., Hardy, S., Neuberg, D., Mulligan, R., Webb, I., Mihm, M., Dranoff, G. (2003). Vaccination With Irradiated, Autologous Melanoma Cells Engineered to Secrete Granulocyte-Macrophage Colony-Stimulating Factor by Adenoviral-Mediated Gene Transfer Augments Antitumor Immunity in Patients With Metastatic Melanoma. JCO 21: 3343-3350 [Abstract] [Full Text]  
  • Lange, P. S., Er, F., Gassanov, N., Hoppe, U. C. (2003). Andersen mutations of KCNJ2 suppress the native inward rectifier current IK1 in a dominant-negative fashion. Cardiovasc Res 59: 321-327 [Abstract] [Full Text]  
  • Sedarous, M., Keramaris, E., O'Hare, M., Melloni, E., Slack, R. S., Elce, J. S., Greer, P. A., Park, D. S. (2003). Calpains Mediate p53 Activation and Neuronal Death Evoked by DNA Damage. J. Biol. Chem. 278: 26031-26038 [Abstract] [Full Text]  
  • Mandinova, A., Soldi, R., Graziani, I., Bagala, C., Bellum, S., Landriscina, M., Tarantini, F., Prudovsky, I., Maciag, T. (2003). S100A13 mediates the copper-dependent stress-induced release of IL-1{alpha} from both human U937 and murine NIH 3T3 cells. J. Cell Sci. 116: 2687-2696 [Abstract] [Full Text]