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J Virol. 1987 March; 61(3): 921-924

Inhibition of Rous sarcoma virus replication by antisense RNA.

L J Chang and C M Stoltzfus

ABSTRACT

Previous results have indicated that Rous sarcoma virus env gene expression is specifically inhibited by antisense RNA (L.-J. Chang and C. M. Stoltzfus, Mol. Cell. Biol. 5:2341-2348, 1985). In this study, we compare the extents of inhibition by antisense RNA derived from different parts of the Rous sarcoma virus genome, and we show that antisense constructs containing the 3'-end noncoding region inhibit env expression to a similar extent as those containing the 5'-end noncoding region or coding region. Furthermore, we show that antisense RNA inhibits virus replication at other levels in addition to translation.

J Virol. 1987 March; 61(3): 921-924




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