This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cai, W Z Articles by DeLuca, N A Search for Related Content PubMed PubMed Citation Articles by Cai, W Z Articles by DeLuca, N A

 Previous Article  |  Next Article 

J Virol. 1987 March; 61(3): 714-721

Linker-insertion nonsense and restriction-site deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1.

ABSTRACT

To study the effects of missense, nonsense, and deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1, a gB-transformed cell line was isolated that, after virus infection, would express sufficient quantities of gB from the cellular chromosome to complement temperature-sensitive gB mutants. The transformed cell line was then used as a permissive cell to transfer two gB mutations from plasmid to viral DNA. One of the mutants, K082, harbored an HpaI linker insertion that introduced one new amino acid and a chain terminator codon within amino acid residue 43. The other mutant contained a 969-base-pair deletion in a part of the gene that includes the membrane-spanning region; a correspondingly shorter gB polypeptide was detected by sodium dodecyl sulfate-gel electrophoresis after immunoprecipitation of infected-cell extracts with four pooled monoclonal antibodies. No polypeptide was observed from K082-infected cells. The shortened gB polypeptide was efficiently processed and secreted into the growth medium. Each of the four monoclonal antibodies precipitated full-length gB, and three of the four precipitated the shortened polypeptide. Enveloped virus particles could be purified after infection of nonpermissive cells with either mutant virus. Virus particles appeared to possess normal polypeptide and glycopeptide profiles except for the absence of gB. Therefore, the presence of gB is not essential for viral assembly, including envelopment. Recombinants in virus stocks grown on the gB-transformed cells occurred at frequencies on the order of 10(-7) to 10(-5), compared with a frequency of approximately 10(-2) in mixed infections with the two mutants.

This article has been cited by other articles:

• Jirmo, A. C., Nagel, C.-H., Bohnen, C., Sodeik, B., Behrens, G. M. N. (2009). Contribution of Direct and Cross-Presentation to CTL Immunity against Herpes Simplex Virus 1. J. Immunol. 182: 283-292 [Abstract] [Full Text]  
• de Jong, M. A. W. P., de Witte, L., Bolmstedt, A., van Kooyk, Y., Geijtenbeek, T. B. H. (2008). Dendritic cells mediate herpes simplex virus infection and transmission through the C-type lectin DC-SIGN. J. Gen. Virol. 89: 2398-2409 [Abstract] [Full Text]  
• Subramanian, R., D'Auvergne, O., Kong, H., Kousoulas, K. G. (2008). The Cytoplasmic Terminus of Kaposi's Sarcoma-Associated Herpesvirus Glycoprotein B Is Not Essential for Virion Egress and Infectivity. J. Virol. 82: 7144-7154 [Abstract] [Full Text]  
• Calistri, A., Sette, P., Salata, C., Cancellotti, E., Forghieri, C., Comin, A., Gottlinger, H., Campadelli-Fiume, G., Palu, G., Parolin, C. (2007). Intracellular Trafficking and Maturation of Herpes Simplex Virus Type 1 gB and Virus Egress Require Functional Biogenesis of Multivesicular Bodies. J. Virol. 81: 11468-11478 [Abstract] [Full Text]  
• Lin, E., Spear, P. G. (2007). Random linker-insertion mutagenesis to identify functional domains of herpes simplex virus type 1 glycoprotein B. Proc. Natl. Acad. Sci. USA 104: 13140-13145 [Abstract] [Full Text]  
• Farnsworth, A., Wisner, T. W., Webb, M., Roller, R., Cohen, G., Eisenberg, R., Johnson, D. C. (2007). Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane. Proc. Natl. Acad. Sci. USA 104: 10187-10192 [Abstract] [Full Text]  
• Mulvey, M., Arias, C., Mohr, I. (2007). Maintenance of Endoplasmic Reticulum (ER) Homeostasis in Herpes Simplex Virus Type 1-Infected Cells through the Association of a Viral Glycoprotein with PERK, a Cellular ER Stress Sensor. J. Virol. 81: 3377-3390 [Abstract] [Full Text]  
• Subramanian, R. P., Geraghty, R. J. (2007). Herpes simplex virus type 1 mediates fusion through a hemifusion intermediate by sequential activity of glycoproteins D, H, L, and B. Proc. Natl. Acad. Sci. USA 104: 2903-2908 [Abstract] [Full Text]  
• Snyder, A., Wisner, T. W., Johnson, D. C. (2006). Herpes Simplex Virus Capsids Are Transported in Neuronal Axons without an Envelope Containing the Viral Glycoproteins. J. Virol. 80: 11165-11177 [Abstract] [Full Text]  
• Farnsworth, A., Johnson, D. C. (2006). Herpes Simplex Virus gE/gI Must Accumulate in the trans-Golgi Network at Early Times and Then Redistribute to Cell Junctions To Promote Cell-Cell Spread.. J. Virol. 80: 3167-3179 [Abstract] [Full Text]  
• Li, W., Minova-Foster, T. J., Norton, D. D., Muggeridge, M. I. (2006). Identification of functional domains in herpes simplex virus 2 glycoprotein B.. J. Virol. 80: 3792-3800 [Abstract] [Full Text]  
• Sloan, D. D., Han, J.-Y., Sandifer, T. K., Stewart, M., Hinz, A. J., Yoon, M., Johnson, D. C., Spear, P. G., Jerome, K. R. (2006). Inhibition of TCR Signaling by Herpes Simplex Virus. J. Immunol. 176: 1825-1833 [Abstract] [Full Text]  
• Bender, F. C., Whitbeck, J. C., Lou, H., Cohen, G. H., Eisenberg, R. J. (2005). Herpes Simplex Virus Glycoprotein B Binds to Cell Surfaces Independently of Heparan Sulfate and Blocks Virus Entry. J. Virol. 79: 11588-11597 [Abstract] [Full Text]  
• Polcicova, K., Goldsmith, K., Rainish, B. L., Wisner, T. W., Johnson, D. C. (2005). The Extracellular Domain of Herpes Simplex Virus gE Is Indispensable for Efficient Cell-to-Cell Spread: Evidence for gE/gI Receptors. J. Virol. 79: 11990-12001 [Abstract] [Full Text]  
• Melancon, J. M., Luna, R. E., Foster, T. P., Kousoulas, K. G. (2005). Herpes Simplex Virus Type 1 gK Is Required for gB-Mediated Virus-Induced Cell Fusion, While neither gB and gK nor gB and UL20p Function Redundantly in Virion De-Envelopment. J. Virol. 79: 299-313 [Abstract] [Full Text]  
• McShane, M. P., Longnecker, R. (2004). Cell-surface expression of a mutated Epstein-Barr virus glycoprotein B allows fusion independent of other viral proteins. Proc. Natl. Acad. Sci. USA 101: 17474-17479 [Abstract] [Full Text]  
• Jiang, C., Wechuck, J. B., Goins, W. F., Krisky, D. M., Wolfe, D., Ataai, M. M., Glorioso, J. C. (2004). Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification. J. Virol. 78: 8994-9006 [Abstract] [Full Text]  
• Beitia Ortiz de Zarate, I., Kaelin, K., Rozenberg, F. (2004). Effects of Mutations in the Cytoplasmic Domain of Herpes Simplex Virus Type 1 Glycoprotein B on Intracellular Transport and Infectivity. J. Virol. 78: 1540-1551 [Abstract] [Full Text]  
• Cheshenko, N., Del Rosario, B., Woda, C., Marcellino, D., Satlin, L. M., Herold, B. C. (2003). Herpes simplex virus triggers activation of calcium-signaling pathways. JCB 163: 283-293 [Abstract] [Full Text]  
• Potel, C., Kaelin, K., Danglot, L., Triller, A., Vannier, C., Rozenberg, F. (2003). Herpes simplex virus type 1 glycoprotein B sorting in hippocampal neurons. J. Gen. Virol. 84: 2613-2624 [Abstract] [Full Text]  
• Milne, R. S. B., Hanna, S. L., Rux, A. H., Willis, S. H., Cohen, G. H., Eisenberg, R. J. (2003). Function of Herpes Simplex Virus Type 1 gD Mutants with Different Receptor-Binding Affinities in Virus Entry and Fusion. J. Virol. 77: 8962-8972 [Abstract] [Full Text]  
• Zhou, G., Roizman, B. (2002). Truncated Forms of Glycoprotein D of Herpes Simplex Virus 1 Capable of Blocking Apoptosis and of Low-Efficiency Entry into Cells Form a Heterodimer Dependent on the Presence of a Cysteine Located in the Shared Transmembrane Domains. J. Virol. 76: 11469-11475 [Abstract] [Full Text]  
• Fan, Z., Grantham, M. L., Smith, M. S., Anderson, E. S., Cardelli, J. A., Muggeridge, M. I. (2002). Truncation of Herpes Simplex Virus Type 2 Glycoprotein B Increases Its Cell Surface Expression and Activity in Cell-Cell Fusion, but These Properties Are Unrelated. J. Virol. 76: 9271-9283 [Abstract] [Full Text]  
• Johnson, D. C., Huber, M. T. (2002). Directed Egress of Animal Viruses Promotes Cell-to-Cell Spread. J. Virol. 76: 1-8 [Full Text]  
• Rodger, G., Boname, J., Bell, S., Minson, T. (2001). Assembly and Organization of Glycoproteins B, C, D, and H in Herpes Simplex Virus Type 1 Particles Lacking Individual Glycoproteins: No Evidence for the Formation of a Complex of These Molecules. J. Virol. 75: 710-716 [Abstract] [Full Text]  
• Mossman, K. L., Macgregor, P. F., Rozmus, J. J., Goryachev, A. B., Edwards, A. M., Smiley, J. R. (2001). Herpes Simplex Virus Triggers and Then Disarms a Host Antiviral Response. J. Virol. 75: 750-758 [Abstract] [Full Text]  
• Desai, P. J. (2000). A Null Mutation in the UL36 Gene of Herpes Simplex Virus Type 1 Results in Accumulation of Unenveloped DNA-Filled Capsids in the Cytoplasm of Infected Cells. J. Virol. 74: 11608-11618 [Abstract] [Full Text]  
• Nixdorf, R., Klupp, B. G., Karger, A., Mettenleiter, T. C. (2000). Effects of Truncation of the Carboxy Terminus of Pseudorabies Virus Glycoprotein B on Infectivity. J. Virol. 74: 7137-7145 [Abstract] [Full Text]  
• Cocchi, F., Menotti, L., Dubreuil, P., Lopez, M., Campadelli-Fiume, G. (2000). Cell-to-Cell Spread of Wild-Type Herpes Simplex Virus Type 1, but Not of Syncytial Strains, Is Mediated by the Immunoglobulin-Like Receptors That Mediate Virion Entry, Nectin1 (PRR1/HveC/HIgR) and Nectin2 (PRR2/HveB). J. Virol. 74: 3909-3917 [Abstract] [Full Text]  
• Wisner, T., Brunetti, C., Dingwell, K., Johnson, D. C. (2000). The Extracellular Domain of Herpes Simplex Virus gE Is Sufficient for Accumulation at Cell Junctions but Not for Cell-to-Cell Spread. J. Virol. 74: 2278-2287 [Abstract] [Full Text]  
• Laquerre, S., Argnani, R., Anderson, D. B., Zucchini, S., Manservigi, R., Glorioso, J. C. (1998). Heparan Sulfate Proteoglycan Binding by Herpes Simplex Virus Type 1 Glycoproteins B and C, Which Differ in Their Contributions to Virus Attachment, Penetration, and Cell-to-Cell Spread. J. Virol. 72: 6119-6130 [Abstract] [Full Text]  
• Laquerre, S., Anderson, D. B., Argnani, R., Glorioso, J. C. (1998). Herpes Simplex Virus Type 1 Glycoprotein B Requires a Cysteine Residue at Position 633 for Folding, Processing, and Incorporation into Mature Infectious Virus Particles. J. Virol. 72: 4940-4949 [Abstract] [Full Text]  
• McNab, A. R., Desai, P., Person, S., Roof, L. L., Thomsen, D. R., Newcomb, W. W., Brown, J. C., Homa, F. L. (1998). The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA. J. Virol. 72: 1060-1070 [Abstract] [Full Text]